¿Es ITS2 más eficaz en la codificación del ADN de angiospermas que las regiones ITS, ITS1, matK y trnH-psbA? Una revisión
DOI:
https://doi.org/10.3989/ajbm.624Palabras clave:
Angiospermas, código de barras de ADN, espaciadores internos transcritos, ITS1, ITS2, cistrón ribosómicoResumen
El espaciador interno transcrito 2 (ITS2) del cistrón ribosómico, que funciona como un minicódigo de barras, ha demostrado ser eficaz en numerosos estudios de código de barras de ADN de angiospermas. Sin embargo, su eficiencia no se ha cuestionado exhaustivamente en la literatura existente. Esta revisión evalúa estadísticamente 197 estudios de código de barras de ADN de angiospermas que utilizaron el minicódigo de barras ITS2. La evaluación abarca no solo la facilidad de amplificación, secuenciación y éxito en la identificación de especies de la región ITS2, sino también una comparación con ITS1, la región ITS completa, matK y trnH-psbA. Los resultados concuerdan con la conclusión previa de que ITS2 puede utilizarse eficazmente para la identificación de angiospermas. Si bien los niveles de amplificación, secuenciación e identificación a nivel de género usando ITS2 fueron altos, el éxito en la identificación a nivel de especie no fue tan prominente. No se observaron diferencias significativas entre los tipos de material o los pares de cebadores utilizados en relación al éxito de la región. El uso exclusivo de ITS2 tuvo un buen rendimiento en comparación con las combinaciones de códigos de barras de ADN. Sin embargo, ninguna de las regiones analizadas puede considerarse un código de barras universal para las angiospermas. Los resultados sugieren que las subregiones ITS1 e ITS2 son igualmente eficaces, seguidas de la región ITS completa, mientras que matK y trnH-psbA no deberían ser las opciones principales.
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