Anales del Jardín Botánico de Madrid, Vol 62, No 2 (2005)

Grapevine yellows diseases in Spain: eight year survey of disease spread and molecular characterization of phytoplasmas involved


https://doi.org/10.3989/ajbm.2005.v62.i2.12

Ester Torres
Laboratori de Sanitat Vegetal, DARP, Generalitat de Catalunya, Barcelona, Spain

Simona Botti
DiSTA, Patologia Vegetale, University of Bologna, Bologna, Italy

Josep Rahola
Servei Sanitat Vegetal, DARP, Generalitat de Catalunya, Spain

María P. Martín
Real Jardín Botánico, Madrid, Spain

Assunta Bertaccini
DiSTA, Patologia Vegetale, University of Bologna, Bologna, Italy

Abstract


Among grapevine yellows phytoplasma diseases in Europe, flavescence dorée (FD) is the most devastating and in the last decade has reached Spanish vineyards, mainly in Catalonia. An eight-year survey was carried out in the areas where the disease has spread (Alt Empordà, Catalonia, Northern Spain) and in the remaining vine-growing areas of Catalonia. Sequence analyses of a portion of the 16S-23S ribosomal DNA cistron, from selected grapevine samples from Catalonia, showed that the phytoplasmas involved in grapevine yellows belong to 16S ribosomal subgroups V-D (flavescence dorée, FD) and XII-A (bois noir, BN). A set of Spanish FD isolates collected during these years were further studied by RFLP analyses of the 16S-23S ribosomal DNA fragment, as well as the rpS3 and SecY genes. All the FD phytoplasma strains studied were related to phytoplasmas belonging to ribosomal protein subgroup rp-E.

Keywords


Flavescence dorée; bois noir; molecular detection; Vitis

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