Overcoming DNA extraction problems from carnivorous plants

Andreas Fleischmann; Günther Heubl

doi:10.3989/ajbm.2198

Autores/as

Andreas Fleischmann LMU Munich, Systematic Botany and Mycology
Günther Heubl LMU Munich, Systematic Botany and Mycology

DOI:

https://doi.org/10.3989/ajbm.2198

Palabras clave:

plantas carnívoras, extracción del ADN, polifenoles, polisacáridos, metabolitos secundarios, mucílago viscoso

Resumen

Probamos algunos protocolos publicados previamente para el aislamiento del ADN de plantas con alto contenido de polifenoles y polisacáridos para varios táxones de plantas carnívoras. Sin embargo, no conseguimos muy buenos resultados ni con tejidos de hojas frescas, ni con tejidos de hojas secadas en gel de sílice obtenidas de plantas colectadas en el campo o cultivadas en los invernaderos, ni de especímenes de herbario. Por lo tanto, hemos desarrollado un protocolo sencillo, modificado del Macherey- Nagel NucleoSpin® Plant kit disponible en el mercado para el aislamiento rápido, eficaz y reproducible de ADN genómico de alta calidad conveniente para la reacción en cadena de la polimerasa. La extracción del ADN se puede realizar en tejidos de hojas frescas o secas de varios táxones de plantas carnívoras, sin importar el grado de contenido de polisacáridos, compuestos fenólicos u otros metabolitos secundarios que interfieren con el aislamiento y la amplificación del ADN.

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Citas

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