Overcoming DNA extraction problems from carnivorous plants

Andreas Fleischmann; Günther Heubl

doi:10.3989/ajbm.2198

Authors

Andreas Fleischmann LMU Munich, Systematic Botany and Mycology
Günther Heubl LMU Munich, Systematic Botany and Mycology

DOI:

https://doi.org/10.3989/ajbm.2198

Keywords:

carnivorous plants, DNA extraction, polyphenols, polysaccharides, secondary metabolites, viscous mucilage

Abstract

We tested previously published protocols for DNA isolation from plants with high contents of polyphenols and polysaccharides for several taxa of carnivorous plants. However, we did not get satisfying results with fresh or silica dried leaf tissue obtained from field collected or greenhouse grown plants, nor from herbarium specimens. Therefore, we have developed a simple modified protocol of the commercially available Macherey- Nagel NucleoSpin® Plant kit for rapid, effective and reproducible isolation of high quality genomic DNA suitable for PCR reactions. DNA extraction can be conducted from both fresh and dried leaf tissue of various carnivorous plant taxa, irrespective of high contents of polysaccharides, phenolic compounds and other secondary plant metabolites that interfere with DNA isolation and amplification.

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