Cytogenetic relationships within the Maghrebian clade of <em>Festuca</em> subgen. <em>Schedonorus</em> (Poaceae), using flow cytometry and FISH

David Ezquerro-López; David Kopecký; Luis Á. Inda

doi:10.3989/ajbm.2455

Autores/as

David Ezquerro-López Grupo BIOFLORA, Escuela Politécnica Superior de Huesca, Instituto Agroalimentario de Aragón–IA2, Universidad de Zaragoza https://orcid.org/0000-0003-1771-8839
David Kopecký Centre of the region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany https://orcid.org/0000-0002-2834-1734
Luis Á. Inda Grupo BIOFLORA, Escuela Politécnica Superior de Huesca, Instituto Agroalimentario de Aragón–IA2, Universidad de Zaragoza https://orcid.org/0000-0002-1214-375X

DOI:

https://doi.org/10.3989/ajbm.2455

Palabras clave:

Festuca subgen. Schedonorus, festucas de hoja ancha, FISH, tamaño genómico, rADN

Resumen

Festuca subgen. Schedonorus es un grupo de Festucas de hojas anchas que se divide tradicionalmente en dos clados, uno europeo y otro magrebí. Mediante hibridación in situ fluorescente —FISH— con sondas específicas para las regiones ribosómicas 5S y 35S en su cariotipo y estimaciones de tamaño genómico mediante citometría de flujo se intentó determinar los posibles genomas parentales de las especies poliploides del clado magrebí. Nuestros datos indican que la especie octoploide F. arundinacea subsp. atlantigena probablemente se originó a partir del cruce de los tetraploides F. arundinacea subsp. fenas —2n = 4x = 28— y F. mairei —2n = 4x = 28— seguido de la duplicación del genoma. Sin embargo, también se ha detectado una reconstrucción del cariotipo y una reducción del tamaño genómico. De forma similar la especie hexaploide F. arundinacea subsp. corsica parece ser un híbrido entre el diploide F. pratensis y el tetraploide F. arundinacea subsp. fenas. Se discuten diversas posibilidades sobre el origen del decaploide F. arundinacea var. letournexiana. Este trabajo contribuye a conocer mejor la filogenia de las Festucas de hoja ancha y proporciona nueva información sobre los cariotipos de estas especies —números cromosomáticos, niveles de ploidía y número y posición de loci de rDNA— mediante FISH y la estima de sus tamaños genómicos mediante citometría de flujo.

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Citas

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