Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH




Broad-leaved fescues, Festuca subgen. Schedonorus, FISH, genome size, rDNA


Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization —FISH— with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing of the tetraploids F. arundinacea subsp. fenas —2n = 4x = 28— and F. mairei —2n = 4x = 28— followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leaved fescues and provided new information on the karyotypes —chromosome numbers, ploidy levels and numbers and positions of rDNA loci— using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.


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Borrill, M. 1972. Studies in Festuca III. The contribution of F. scariosa to the evolution of polyploids in sections Bovinae and Scariosae. New Phytologist 71: 523-532.

Borrill, M., Kirby, M. & Morgan, W.G. 1977. Studies in Festuca 11. Interrelationships of some putative diploid ancestors of the polyploid broad-leaved fescues. New Phytologist 78: 661-674.

Catalán, P., Torrecilla, P., Rodríguez, J.A.L. & Olmstead, R.G. 2004. Phylogeny of the festucoid grasses of subtribe Loliinae and allies (Poeae, Pooideae) inferred from ITS and trnL-F sequences. Molecular Phylogenetics and Evolution 31: 517-541. PMid:15062792

Catalán, P. 2006. Phylogeny and evolution of Festuca L. and related genera of subtribe Loliinae (Poeae, Poaceae). In: Sharma, A.K. & Sharma, A. (eds.), Plant Genome. Biodiversity and Evolution. Pp. 255- 303. Enfield: Science Publishers.

Chandrasekharan, P. & Thomas, H. 1971. Studies in Festuca. V. Cytogenetic relationships between species of Bovinae and Scariosae. Zeitschrift für Pflanzenzuchtung 65: 353-354.

Charmet, G., Ravel, C. & Balfourier, F. 1997. Phylogenetic analysis in the Festuca-Lolium complex using molecular markers and ITS rDNA. Theoretical and Applied Genetics 94: 1038-1046.

Dolezel, J., Greilhuber, J., Lucretti, S., Meister, A., Lysak, M., Nardi, L. & Obermayer, R. 1998. Plant genome size estimation by flow cytometry: interlaboratory comparison. Annals of Botany 82: 17-26.

Hackel, E. 1882. Monografia Festucarum Europearum. Kassel and Berlin: T. Fischer.

Hammatt, N., Blackall, N.W. & Davey, M.R. 1991. Variation in the DNA content of Glycine species. Journal of Experimental Botany 42: 659-665.

Hand, M., Cogan, N.O.I., Stewar,t A.V. & Forster, J.W. 2010. Evolutionary history of tall fescue morphotypes inferred from molecular phylogenetics of the Lolium-Festuca species complex. BMC Evolutionary Biology 10: 303-320. PMid:20937141 PMCid:PMC2958922

Harper, J.A., Thomas, I.D., Lovatt, J.A. & Thomas, H.M. 2004. Physical mapping of rDNA sites in possible diploid progenitors of polyploid Festuca species. Plant Systematics and Evolution 245: 163-168.

Humphreys, M.W., Thomas, H.M., Morgan, W.G., Meredith, M.R., Harper, J.A., Thomas, H., Zwierzykowski, Z. & Ghesquiére, M. 1995. Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization. Heredity 75: 171-174.

Huska, D., Leitch, I.J., De Carvalho, J.F., Leitch, A.R., Salmon, A., Ainouche, M. & Kovarik, A. 2016. Persistence, dispersal and genetic evolution of recently formed Spartina homoploid hybrids and allopolyploids in Southern England. Biological Invasions 18: 2137-2151.

Inda, L.Á., Segarra-Moragues, J.G., Müller, J., Peterson, P.M. & Catalán, P. 2008. Dated historical biogeography of the temperate Loliinae (Poaceae, Pooideae) grasses in the northern and southern hemispheres. Molecular Phylogenetics and Evolution 46: 932-957. PMid:18226932

Inda, L. Á., Sanmartín, I., Buerki, S. & Catalán, P. 2014. Mediterranean origin and Miocene-Holocene Old World diversification of meadow fescues and ryegrasses (Festuca subgenus Schedonorus and Lolium). Journal of Biogeography 41: 600-614.

Inda, L. Á. & Wolny, E. 2013. Fluorescent in situ hybridization of the ribosomal RNA genes (5S and 35S) in the genus Lolium: Lolium canariense, the missing link with Festuca? Anales del Jardín Botánico de Madrid 70: 97-102.

Jenkins, G. & Hasterok, R. 2007. BAC ´landing’on chromosomes of Brachypodium distachyon for comparative genome alignment. Nature Protocols 2: 88-98. PMid:17401342

Kopecky, D., Lukaszewski, A.J. & Dole?el, J. 2005. Genomic constitution of Festulolium cultivars released in the Czech Republic. Plant Breeding 124: 454-458.

Kopecky, D., Lukaszewski, A.J. & Dole?el, J. 2008a. Cytogenetics of Festulolium (Festuca ? Lolium hybrids). Cytogenetics and Genome Research 120: 370-383. PMid:18504366

Kopecky, D., Lukaszewsk, A.J. & Dole?el, J. 2008b. Meiotic behavior of individual chromosomes of Festuca pratensis in tetraploid Lolium multiflorum. Chromosome Research 16: 987-998. PMid:18830677

Kopeck?, D., Havránková, M., Loureiro, J., Castro, S., Lukaszewski, A.J., Barto?, J., Kopecká, J. & Dole?el, J. 2010. Physical distribution of homoeologous recombination in individual chromosomes of Lolium multiflorum/Festuca pratensis substitutions. Cytogenetic and Genome Research 129: 162-172.

Ksiazczyk, T., Taciak, M. & Zwierzykowski, Z. 2010. Variability of ribosomal DNA sites in Festuca pratensis, Lolium perenne, and their intergeneric hybrids, revealed by FISH and GISH. Journal of Applied Genetics 51: 449-60. PMid:21063062

Leggett, J.M. & Markhand, G.S. 1995. The genomic identification of some monosomics of Avena sativa L-Cv Sun-Ii using genomic in situ hybridization. Genome 38: 747-751. PMid:18470201

Leitch, I.J. & Bennett, M.D. 2004. Genome downsizing in polyploid plants. Biological Journal of the Linnean Society 82: 651-663.

Lideikyte, L., Pasakinskiene, I., Lemeziene, N., Nekrosas, S. & Kanapeckas, J. 2008. FISH assessment of ribososmal DNA sites in the chromosome sets of Lolium, Festuca and Festuloium. Zemdirbyste 95: 116-124.

Loureiro, J., Kopeck?, D., Castro, S., Santos, C. & Silveira, P. 2007. Flow cytometric and cytogenetic analyses of Iberian Peninsula fescues. Plant Systematics and Evolution 269: 89-105.

Malik, C.P. & Thomas, P.T. 1966. Kariotypic studies in some Lolium and Festuca species. Caryologia 19: 167-196.

Ohri, D., Fritsch, R.M. & Hanelt, P. 1998. Evolution of genome size in Allium (Alliaceae). Plant Systematics and Evolution 210: 57-86.

Otto, F. 1992. Preparation and staining of cells for high-resolution DNA analysis. In: Radbruch A. (ed.), Flow cytometry and cell sorting. Pp. 65-68. Berlin: Springer-Verlag.

Ozkan, H., Tuna, M. & Arumuganathan, K. 2003. Nonadditive Changes in genome Size During Allopolyploidization in the Wheat (Aegilops- Triticum) Group. Journal of Heredity 94: 260-264. PMid:12816968

Rees, H. & Walters, M.R. 1965. Nuclear DNA and the evolution of wheat. Heredity 20: 73-82.

Shaked, H., Kashkush, K., Ozkan, H., Feldman, M. & Levy, A.A. 2001. Sequence elimination and cytosine methylation are rapid and reproducible responses of the genome to wide hybridization and allopolyploidy in wheat. Plant Cell 13: 1749-1759. PMid:11487690 PMCid:PMC139131

Sing, K.P., Raina, S.N. & Singh, A.K. 1996. Variation in chromosomal DNA associated with the evolution of Arachis species. Genome 39: 890-897.

?marda, P., Bures, P., Horova, L., Foggi, B. & Rossi, G. 2008. Genomic Size and GC Content Evolution of Festuca: Ancestral Expansion and Subsequent Reduction. Annals of Botany 101: 421-433.

Thomas, H.M., Harper, J.A., Meredith, M.R., Morgan, W.G. & King, I.P. 1997. Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization. Genome 40: 406-410. PMid:18464836

Thomas, H.M., Harper, J.A., Meredith, M.R., Morgan, W.G., Thomas, I.D., Timms, E. & King, I.P. 1996. Comparison of ribosomal DNA sites in Lolium species by fluorescence in situ hybridization. Chromosome Research 4: 486-490. PMid:8939359

Thomas, H.M., Morgan, W.G., Meredith, M.R., Humphreys, M.W., Thomas, H. & Leggett, J.M. 1994. Identification of parental and recombined chromosomes in hybrid derivatives of Lolium multiflorum x Festuca pratensis by genomic in situ hybridization. Theoretical and Applied Genetics 88: 909-13. PMid:24186241

Torrecilla, P. & Catalán, P. 2002. Phylogeny of Broad-leaved and Fine-leaved Festuca Lineages (Poaceae) based on Nuclear ITS Sequences. Systematic Botany 27: 241-251.

Valladolid, A., Blas, R. & Gonzáles, R. 2004. Introducción al recuento de cromosomas somáticos en raíces andinas. In: Seminario, J. (ed.), Conservación y uso de la biodiversidad de raíces y tubérculos andinos: Una década de investigación para el desarrollo (1993-2003). Pp. 95-99. Lima: Centro Internacional de la Papa.

Vaughan, H.E., Jamilena, M., Rejon, C.R., Parker, J.S. & Garridoramos, M.A. 1993. Loss of nucleolar-organizer regions during polyploid evolution in Scilla autumnalis. Heredity 71: 574-580.



How to Cite

Ezquerro-López, D., Kopecký D., & Inda, L. Á. (2017). Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH. Anales Del Jardín Botánico De Madrid, 74(1), e052.