A study of seed micromorphology in the genus Ophrys (Orchidaceae)


  • Pablo Galán Cela Departamento de Producción Vegetal, Botánica y Protección Vegetal, Universidad Politécnica de Madrid
  • Irene Seligrat Departamento de Biología, Universidad Autónoma de Madrid
  • Emma Ortúñez Departamento de Biología, Universidad Autónoma de Madrid
  • Roberto Gamarra Departamento de Biología, Universidad Autónoma de Madrid
  • Alejandro Vivar Departamento de Ciencias Básicas Aplicadas a la Ingeniería Forestal, Universidad Politécnica de Madrid
  • Antonio Scrugli Dipartimento di Scienze Botaniche, Università di Cagliari




testa cells, periclinal walls, SEM, statistical analysis


Seed micromorphology of 19 taxa of the genus Ophrys have been studied using SEM and light microscope. Quantitative data (length and width of seed and embryo, number of testa cells along the longitudinal axis, volume of seed and embryo, and percentage of free air space), as well as qualitative characters (seed shape, features of the anticlinal and periclinal walls, ornamentation and colour) were analysed. All the seeds are fusiform, with an asymmetrical basal pole, the periclinal walls of the medial cells have parallel and transverse to slanting ridges, and raised anticlinal walls. Statistical analyses show two large clusters according to the volumes of seed and embryo. Our results support the monophyly of the genus and their recent diversification, however, seed features are not congruent with the recognition of sections and groups within Ophrys.


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Amich, F., García-Barriuso, M. & Bernardos, S. 2007. Polyploidy and speciation in the orchid flora of the Iberian Peninsula. Botanica Helvetica 117: 143-157. http://dx.doi.org/10.1007/s00035-007-0804-0

Arditti, J., Michaud, J.D. & Healey, P.L. 1979. Morphometry of orchid seeds. I. Paphiopedilum and native California and related species of Cypripedium. American Journal of Botany 66: 1128-1137. http://dx.doi.org/10.2307/2442211

Arditti, J. & Ghani, A.K.A. 2000. Numerical and physical properties of orchid seeds and their biological implications. New Phytologist 145: 367-421. http://dx.doi.org/10.1046/j.1469-8137.2000.00587.x

Averyanov, L.V. 1990. A review of the genus Dactylorhiza. In: Arditti, J. (ed.), Orchid Biology. Reviews and Perspectives. 5: 159-206. Timber Press, Portland.

Aybeke, M. 2007. Pollen and seed morphology of some Ophrys L. (Orchidaceae) taxa. Journal of Plant Biology 50: 387-395. http://dx.doi.org/10.1007/BF03030673

Barthlott, W. 1976. Morphologie der Samen von Orchideen im Hinblick auf taxonomische und funktionelle Aspekte. In: Senghas, K. (ed.), Proceedings of the 8th World Orchid Conference Frankfurt 444-455. Parey, Hamburg. PMid:972720

Barthlott, W. & Ziegler, B. 1981. Mikromorphologie der Samenschalen als systematisches Merkmal bei Orchideen. Berichte der Deutschen Botanischen Gesellschaft 94: 267-273.

Bateman, R.M., Hollingsworth, P.M., Preston, J., Yi-bo, L., Pridgeon, A.M., Chase, M.W. 2003. Molecular phylogenetics and evolution of Orchidinae and selected Habenariinae (Orchidadeae). Botanical Journal of the Linnean Society 142: 1-40. http://dx.doi.org/10.1046/j.1095-8339.2003.00157.x

Baumann, H. & Ku.nkele, S. 1986. Die Gattung Ophrys L. – eine taxonomische Übersicht. Mitteilungen Arbeitskreis Heimische Orchideen Baden-Wu.rttemberg 16: 633-663.

Bernardos, S., Crespí, A., Del Rey, F. & Amich, F. 2005. The section Pseudophrys (Ophrys, Orchidaceae) in the Iberian Peninsula: a morphometric and molecular analysis. Botanical Journal of the Linnean Society 148: 359-375. http://dx.doi.org/10.1111/j.1095-8339.2005.00403.x

Clifford, H.T. & Smith, W.K. 1969. Seed morphology and classification of Orchidaceae. Phytomorphology 19: 133-139.

D'Emerico, S., Pignone, D., Bartolo, G., Pulvirenti, S., Terrasi, C., Stuto, S. & Scrugli, A. 2005. Karyomorphology, heterochromatin patterns and evolution in the genus Ophrys (Orchidaceae). Botanical Journal of the Linnean Society 148: 87-99. http://dx.doi.org/10.1111/j.1095-8339.2005.00393.x

Del Prete, C. 1984. The genus Ophrys L. (Orchidaceae): a new taxonomic approach. Webbia 38: 209-220. http://dx.doi.org/10.1080/00837792.1984.10670307

Delforge, P. 2006. Orchids of Europe, North Africa and the Middle East, 3rd ed. A&C Black, London.

Devillers, P. & Devillers-Terschuren, J. 1994. Essai d'analyse systématique du genre Ophrys. Naturalistes Belges (Orchid. 7, suppl.) 75: 273-400.

Gamarra, R., Dorda, E., Scrugli, A., Galán, P. & Ortúñez, E. 2007. Seed micromorphology in the genus Neotinea Rchb.f. (Orchidaceae, Orchidinae). Botanical Journal of the Linnean Society 153: 133-140. http://dx.doi.org/10.1111/j.1095-8339.2006.00603.x

Gamarra, R., Galán, P., Herrera, I. & Ortúñez, E. 2008. Seed micromorphology supports the splitting of Limnorchis from Platanthera (Orchidaceae). Nordic Journal of Botany 26: 61-65. http://dx.doi.org/10.1111/j.1756-1051.2008.00135.x

Gamarra, R., Ortúñez, E., Galán Cela, P. & Guadaño, V. 2012. Anacamptis versus Orchis (Orchidaceae): seed micromorphology and its taxonomic significance. Plant Systematics and Evolution 298: 597-607. http://dx.doi.org/10.1007/s00606-011-0569-1

Godfery, M.J. 1928. Classification of the genus Ophrys. Journal of Botany 66: 33-36.

Greilhuber, J. & Ehrendorfer, F. 1975. Chromosome numbers and evolution in Ophrys (Orchidaceae). Plant Systematics and Evolution 124: 125-138. http://dx.doi.org/10.1007/BF00985498

Inda, L.A., Pimentel, M. & Chase, M.W. 2010. Contribution of mitocondrial cox1 intron sequences to the phylogenetics of tribe Orchideae (Orchidaceae): Do the distribution and sequence of this intron in orchids also tell us something about its evolution? Taxon 59(4): 1053-1064.

Inda, L.A., Pimentel, M. & Chase, M.W. 2012. Phylogenetics of tribe Orchideae (Orchidaceae; Orchidoideae) based on combined DNA matrices: inferences regarding timing of diversification and evolution of pollination syndromes. Annals of Botany 110: 71-90. http://dx.doi.org/10.1093/aob/mcs083 PMid:22539542 PMCid:PMC3380586

Molvray, M. & Kores, P.J. 1995. Character analysis of the seed coat in Spiranthoideae and Orchidoideae, with special reference to the Diurideae (Orchidaceae). American Journal of Botany 82: 1443-1454. http://dx.doi.org/10.2307/2445872

Mrkvicka, A.C. 1994. Anatomie und Morphologie der Samen heimischer Orchideenarten. Journal Europäischer Orchideen 26: 168-314.

Nelson, E. 1962. Gestaltwandel und Artbildung erörtert am Beispiel der Orchidaceen Europas und der Mittelmeerländer insbesondere der Gattung Ophrys – mit einer Monographie und Ikonographie der Gattung Ophrys. Verlag E. Nelson, Chernex-Montreux.

Paulus, H.F. & Gack, C. 1990. Pollinators as prepollinating isolation factors: evolution and speciation in Ophrys (Orchidaceae). Israel Journal of Botany 39: 43-79.

Pedersen, H.A. & Faurholdt, N. 2007. Ophrys. The bee orchids of Europe. Kew Publishing, Royal Botanic Gardens, Kew.

Pridgeon, A.M., Cribb, P.J., Chase, M.W. & Rasmussen, F.N. 2001. Genera Orchidacearum. Volume 2. Orchidoideae (part one). Oxford University Press, Oxford. PMid:21669661

Soliva, M., Kocyan, A. & Widmer, A. 2001. Molecular phylogenetics of the sexually deceptive orchid genus Ophrys (Orchidaceae) based on nuclear and chloroplast DNA sequences. Molecular Phylogenetics and Evolution 20: 78-88. http://dx.doi.org/10.1006/mpev.2001.0953 PMid:11421649

Thiers, B. 2012. Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden's Virtual Herbarium. http://sweetgum.nybg.org/ih/ [continuously updated; accessed September 2012].

Tohda, H. 1983. Seed morphology in Orchidaceae I. Dactylorchis, Orchis, Ponerorchis, Chondradenia and Galeorchis. Science Reports of the Tohoku University, 4th ser., Biology 38: 253-268.




How to Cite

Galán Cela, P., Seligrat, I., Ortúñez, E., Gamarra, R., Vivar, A., & Scrugli, A. (2014). A study of seed micromorphology in the genus Ophrys (Orchidaceae). Anales Del Jardín Botánico De Madrid, 71(2), e008. https://doi.org/10.3989/ajbm.2370




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