TY - JOUR AU - Torres, Ester AU - Botti, Simona AU - Rahola, Josep AU - Martín, María P. AU - Bertaccini, Assunta PY - 2005/12/30 Y2 - 2024/03/28 TI - Grapevine yellows diseases in Spain: eight year survey of disease spread and molecular characterization of phytoplasmas involved JF - Anales del Jardín Botánico de Madrid JA - An. Jard. Bot. Madr. VL - 62 IS - 2 SE - Articles DO - 10.3989/ajbm.2005.v62.i2.12 UR - https://rjb.revistas.csic.es/index.php/rjb/article/view/12 SP - 127-133 AB - Among grapevine yellows phytoplasma diseases in Europe, flavescence dorée (FD) is the most devastating and in the last decade has reached Spanish vineyards, mainly in Catalonia. An eight-year survey was carried out in the areas where the disease has spread (Alt Empordà, Catalonia, Northern Spain) and in the remaining vine-growing areas of Catalonia. Sequence analyses of a portion of the 16S-23S ribosomal DNA cistron, from selected grapevine samples from Catalonia, showed that the phytoplasmas involved in grapevine yellows belong to 16S ribosomal subgroups V-D (flavescence dorée, FD) and XII-A (bois noir, BN). A set of Spanish FD isolates collected during these years were further studied by RFLP analyses of the 16S-23S ribosomal DNA fragment, as well as the rpS3 and SecY genes. All the FD phytoplasma strains studied were related to phytoplasmas belonging to ribosomal protein subgroup rp-E. ER -