@article{Torres_Botti_Rahola_Martín_Bertaccini_2005, title={Grapevine yellows diseases in Spain: eight year survey of disease spread and molecular characterization of phytoplasmas involved}, volume={62}, url={https://rjb.revistas.csic.es/index.php/rjb/article/view/12}, DOI={10.3989/ajbm.2005.v62.i2.12}, abstractNote={Among grapevine yellows phytoplasma diseases in Europe, flavescence dorée (FD) is the most devastating and in the last decade has reached Spanish vineyards, mainly in Catalonia. An eight-year survey was carried out in the areas where the disease has spread (Alt Empordà, Catalonia, Northern Spain) and in the remaining vine-growing areas of Catalonia. Sequence analyses of a portion of the 16S-23S ribosomal DNA cistron, from selected grapevine samples from Catalonia, showed that the phytoplasmas involved in grapevine yellows belong to 16S ribosomal subgroups V-D (flavescence dorée, FD) and XII-A (bois noir, BN). A set of Spanish FD isolates collected during these years were further studied by RFLP analyses of the 16S-23S ribosomal DNA fragment, as well as the rpS3 and SecY genes. All the FD phytoplasma strains studied were related to phytoplasmas belonging to ribosomal protein subgroup rp-E.}, number={2}, journal={Anales del Jardín Botánico de Madrid}, author={Torres, Ester and Botti, Simona and Rahola, Josep and Martín, María P. and Bertaccini, Assunta}, year={2005}, month={Dec.}, pages={127–133} }