AJBM Anales del Jardín Botánico de Madrid 0211-1322 1988-3196 Consejo Superior de Investigaciones Cientificas AJBM201809-2495 10.3989/ajbm.2495 Articles Euphorbia duvalii (Euphorbiaceae) in the Iberian Peninsula Euphorbia duvalii Lecoq & Lamotte en la península ibérica Euphorbia duvalii (Euphorbiaceae) in the Iberian Peninsula Bou Jordi 1 * Tomàs Josep Bou i 2 LAGP-Flora and Vegetation, Institute of the Environment, University of Girona, Campus Montilivi, 17071 Girona, Spain HGI, University of Girona, Campus Montilivi, 17071 Girona, Spain Author for correspondence: jordi.bou.manobens@gmail.com, https://orcid.org/0000-0001-9454-8023 pistacia400@gmail.com, https://orcid.org/0000-0001-5043-5318

Associate Editor: M. Velayos.

 19 11 2018 2018 75 2 10.3989/ajbm.2495 18 04 2018 1 10 2018 © 2018 CSIC 2018 This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial (by-nc) Spain 4.0 License. Abstract

Euphorbia duvalii Lecoq & Lamotte is an endemic species from the Catalan-Occitan territory ―western Mediterranean area― and is usually found in the south of France. Euphorbia duvalii has been cited from some localities of the Iberian Peninsula, but in later reviews all of these citations were finally identified as other species of Euphorbia L. One of these citations, from the Alt Empordà ―Girona, Spain―, was of specific interest because not all the sources agreed on what the species was. To clarify whether or not this species is really present in the Iberian Peninsula, we examined new material from this population in order to establish whether the species is in fact E. duvalii. Once the morphology of the specimens from the Serra de Bac Grillera population had been analysed, we were able to confirm that they were indeed E. duvalii. This means that this is the only area on the Iberian Peninsula where this endemic species has been identified. The population is located in a rocky meadow that contains some scrub and is surrounded by pinewoods. Because this is the one and only Iberian population, a management and conservation plan needs to be drawn up to ensure its preservation.

 Resumen

Euphorbia duvalii Lecoq & Lamotte es una especie endémica del territorio catalano-occitano ―área mediterránea occidental― que puede ser hallada normalmente en el sur de Francia. Euphorbia duvalii ha sido citada de algunas localidades de la Península Ibérica, pero en revisiones posteriores todas las citas se identificaron finalmente como otras especies de Euphorbia L. Una de estas citas, la del Alt Empordà ―Gerona, España―, era muy interesante, ya que no todas las fuentes estaban de acuerdo a qué especie correspondía. Para clarificar si esta especie se encuentra en la Península Ibérica, examinamos material nuevo de esta localidad para determinar si la especie era de hecho E. duvalii. Tras el análisis de la morfología de los especímenes de la población de la sierra de Bac Grillera, fuimos capaces de confirmar que se trataba de E. duvalli. Esto significa que ésta es la única área en la península ibérica donde esta especie endémica ha sido identificada. La población se encuentra en un prado rocoso con algunos matorrales y rodeado de pinares. Ya que ésta es la única población ibérica, es necesario un plan de gestión y conservación de esta especie para su preservación.

 Keywords Alt Empordà Alta Garrotxa conservation singular flora protected species Serra de Bac Grillera Palabras clave Alto Ampurdán Alta Garrotxa conservación especies protegidas flora singular sierra de Bac Grillera INTRODUCTION

Euphorbia duvalii Lecoq & Lamotte (Euphorbiaceae Juss.) is an endemic plant from the Catalan-Occitan territory, of which there are a few populations in the southeast of France where the plants can be found in the rocky calcicolous meadows in the Midi Mountains: Causses des Cévennes, Causses du Quercy, Corbières, and Pyrénées-Orientales in the departments of Ardèche, Aude, Aveyron, Gard, Hérault and Lozère (Simon & Vicens 1999; Tison & al. 2014). However, in the Iberian Peninsula it is known to have only one habitat in the northeast of Catalonia, in the Alt Empordà, in the wilderness area of the Alta Garrotxa (Bou i Tomàs 1985). Although there are citations from the Serra del Boumort (Molero & al. 1988; Pujadas 1981) and Montsec (Romo 1989), these would appear in fact to be referring to E. dulcis L. (Simon & Vicens 1999).

There have been several incidences of confusion about the presence of this taxon on the Iberian Peninsula. The Iberian Flora collection refers to the Alt Empordà population as corresponding to E. angulata Jacq. (Benedí & al., 1997), while other studies confirm the presence of E. duvalii in the Iberian Peninsula (Simon & Vicens 1999). At the same time, the Flora Manual dels Països Catalans, ed. 3 describes a doubtful presence of this taxon in the territori ruscínic ―Ruscinicum― (Bolòs & al. 2005).

With only one population on the Iberian Peninsula, this unclear situation regarding the current status of E. duvalii has generated a serious problem in terms of knowledge about and conservation of this Mediterranean plant. Because of its rarity, E. duvalii was declared as strictly protected in Catalonia under the Pla d’Espais d’Interès Natural (Generalitat de Catalunya 1993), which was then later modified through the creation of the Catàleg de la Flora Amenaçada a Catalunya (Generalitat de Catalunya 2008), which states this taxon as being a strictly protected species of flora in the wilderness area of the Alta Garrotxa. Therefore, in this study we wanted to check for the presence of this species in the Alt Empordà through field work and by analysing the herbarium material while, at the same time, contributing to the knowledge about this plant by adding new chorological data about the one and only population on the Iberian Peninsula. We also endeavour to provide clarity and useful information to ensure the preservation of this endemism of occidental Mediterranean flora.

 MATERIAL AND METHODS

The studied population of E. duvalii is found in the eastern part of the Pre-Pyrenees mountain range known as the Serra de Bac Grillera. It is inside the protected area of the Alta Garrotxa which is included in the Pla d’Espais d’Interès Natural and also the Xarxa Natura 2000 (fig. 1). Material was collected to carry out a morphological study, and the specimens were later deposited in the Herbari de la Universitat de Girona ―HGI―. An identification key was constructed from the data collected, together with the bibliographic information from Simon & Vicens (1999) and Tison & al. (2014). All the studied specimens of Euphorbia are listed in the appendix.

Populations of E. duvalii Lecoq & Lamotte in the western Mediterranean, and in detail in the locality of the Serra de Bac Grillera; the known localities for field work and bibliographic citations are represented. [Prepared by the authors; sources: Generalitat de Catalunya (2017); Institut Cartogràfic i Geològic de Catalunya (2017); Tela Botanica (2017)].

The site studied is an open wilderness space made up of shrub lands and meadows (fig. 2c) and surrounded by a Scots pine forest. The ground is stony with disaggregated red soils that have signs of a marked erosive processes. As with the French populations, the substrate is calcareous (Tison & al. 2014) and more specifically in the Iberian case there are calcareous rocks from Cretaceous and Garumnian facies. The population is located 800 m a.s.l. on a north-east facing site which produces the specific environmental conditions of sub-Mediterranean limestone mountains.

Euhorbia duvalii Lecoq & Lamotte: a, complete plant with woody rhizomes; b, habitat where the Iberian population grows in the Serra de Bac Grillera; c, capsule with sub-pyramidal and sub-conical warts [a, c, HGI 23815].

 RESULTS AND DISCUSSION Morphology

The specimens of E. duvalii collected in the Serra de Bac Grillera ―HGI 23815 and HGI 23816― correspond to a perennial herbaceous plant and are usually glabrous and multicauline. Stems are 10–20(25) cm long, ascending and mostly simple and cylindrical. Woody rhizomes without tuberculous structures are between a dark brown and greyish colour and unarticulated. Leaves are of variable morphology. There are numerous basal cataphylls 2–2.5 × 1.5–2 mm which are scarious, obtuse and with a rounded apex. Cauline leaves are [8]10–20[36] × [2.5]3–6[9.5] mm, firm, sessile and alternate, lanceolate to elliptic in shape, and denticulate in the upper half. Leaves from sterile stems tend to be finely lanceolate and more acute towards the apex. The pleiochasial bracts are [7]8–10[19] × [7]4–8[10] mm, ovate to rhombic in shape which is often quite a lot shorter than the radius. Dichasial bracts are [6]4–5[9] × [7]6–8[14]mm and oblate in shape with a mucronate apex. They are pleiochasium, green, with a 3–5 floral radius, albeit sometimes we were able to observe an axillar floral radius. Nectar glands are a reniform shape and yellow in colour. The sub-globular capsules are [3.3]3.6–4[4.8] mm in diameter, glabrous and green but the mature capsules are red-brown with warts visible to the naked eye. The warts are [0.8]0.7–1.2[1.1] mm long, and although they are thick, they are clearly longer than they are wide, and are sub-pyramidal to sub-conical. The style is long, trifurcated in the middle of the length and persistent to maturity. Seeds are sub-globular with a truncated base and a subacute 2.5 × 2 mm apex, which is smooth and dark brown. They are reniform caruncle with an apparent frontal notch.

All these characteristics lead us to conclude that there is a population of E. duvalii in the Serra de Bac Grillera as the morphology of the specimens collected corresponds to the descriptions of Simon & Vicens (1999) and Tison & al. (2014). The same is observed for the samples collected in 1983 ―HGI 23847―, which also correspond to E. duvalii. Furthermore, we have compared the material we collected with the French specimens of E. duvalii from Mont Alaric ―HGI 23817― and with herbarium material ―BC 649310 and BC 649337―, and the same characteristics are clearly identifiable.

In addition, samples of other species ―E. dulcis and E. angulata― that could be confused with E. duvalii have been analysed. As has been observed, with the complete sample of E. angulata ―BC 149063, BC 149079, HGI 23850, and HGI 23851―, the rhizome with nodules that this taxon presents can be clearly seen, and so cannot be confused with the woody rhizome of E. duvalii. However the differences between the rhizomes of E. dulcis ―BC 651571 and BC 917673― and E. duvalii are not obvious at first sight, and so the capsules need to be analysed, because E. dulcis has a lower density of warts than does E. duvalii and they are also shorter.

To clarify these differences, the following identification key has been developed:

1. Woody and unarticulated rhizomes. Sub-globular capsules with 0.7–1.2 mm long sub-pyramidal to sub-conical warts .......... E. duvalii Lecoq & Lamotte
– Fleshy and articulated rhizomes. Sub-globular capsules with 0.4–0.7 mm long sub-conical warts .................. 2
2. Rhizomes with continuous segments …....… E. dulcis L.
– Rhizomes with discontinuous nodules .................................................................................... E. angulata Jacq.
State of conservation

The population of E. duvalii in the Serra de Bac Grillera is unique to the Iberian Peninsula and, due to its restricted distribution in France, its conservation is of great importance. However, the possible presence in other localities in the northeast of the Iberian Peninsula cannot be discarded. To preserve this unique element of western Mediterranean flora, knowledge concerning the biology and ecology of this species needs to be extended and an efficient management plan needs to be developed.

 ACKNOWLEDGEMENTS

The authors would like to thank R.M. Manobens for her assistance in collecting the plants and Dr. L. Vilar from the LAGP-Flora and vegetation (UdG) for his timely and valuable advice.

 REFERENCES Benedí C. Molero J. Simon J. Vicens J. 1997 Euphorbia L Castroviejo S. al Flora iberica 8 210 285 CSIC Madrid Bolòs O. Vigo J. Masalles R.M. Ninot J. M. 2005 Flora Manual dels Països Catalans 3 Pòrtic Barcelona Bou i Tomàs J. Noves dades florístiques per a la flora de l’Alt Empordà (NE de Catalunya). II Collectanea Botanica 1985 16 1 165 173 Generalitat de Catalunya Decret 328/1992, de 14 de desembre, pel qual s’aprova el Pla d’espais d’interès natural Diari Oficial de la Generalitat de Catalunya 1993 1714 1 17 Generalitat de Catalunya Decret 172/2008, de 26 d’agost, de creació del Catàleg de flora amenaçada de Catalunya Diari Oficial de la Generalitat de Catalunya 2009 5204 65881 65896 Generalitat de Catalunya gencat.cat 2017 Website: http://www.gencat.cat/ [accessed 15–X–2017] Institut Cartogràfic i Geològic de Catalunya ICGC VISSIR 2017 Website: http://www.icc.cat [accessed 15–X–2017] Molero J. Pujadas J. Romo A.M. Noves dades corològiques i taxonòmiques sobre la Flora dels Prepirineus centrals catalans Monográfico Instituto Pirenaico de Ecología 1988 4 265 281 Pujadas J Algunes plantes del Boumort Butlletí de la Institució Catalana d’Història Natural 1981 46 4 101 102 Romo A.M. 1989 Flora i Vegetació del Montsec Pre-Pirineus catalans IEC Barcelona Simon J. Vicens J. 1999 Estudis biosistemàtics en Euphorbia L. a la Mediterrània occidental IEC Barcelona Tela Botanica Fiche eFlore de Euphorbia duvali 2017 Website: http://www.tela-botanica.org [accessed 15–X–2017] Tison J.-M. Jauzein P. Michaud H. 2014 Flore de la France Méditerranéenne Continentale Naturalia Publications Turriers APPENDIX

Euphorbia duvalii Lecoq & Lamotte

FRANCE. Aude: Comingne, Mont Alaric (Corbières), 23–VII–2017, J. Bou, R.M. Manobens and J. Bou Manobens s.n. leg. (HGI 23817); Gard, Fontaret, près de Blandas, 21–VI–1880, L. Anthonard s.n. leg. (BC 649310). Unknown department: Pert, V–1889, L. Giraudias s.n. leg. (BC 649337).

SPAIN. Girona: Alt Empordà, Maçanet de Cabrenys, a la Serra de Bac Grillera, a la Collada dels Gesos, 31TDG7689, 750 m a.s.l., 24–VI–2017, J. Bou Manobens, J. Bou i Tomàs and R.M. Manobens s.n. leg. (HGI 23815, HGI 23816); Alt Empordà, Maçanet de Cabrenys, calcàries del Castell de Bac Grillera, DG79, 750 m a.s.l., 05–VI–1983, J. Bou i Tomàs s.n. leg. (HGI 23847).

Euphorbia dulcis L.

SPAIN. Lleida: Alt Urgell, la Guàrdia d’Ares, Barranc Fondo, 10–VII–1980, J. Molero and J. Pujadas s.n. leg. (BC 651571). Asturias: Oviedo, Monte Naranco, 30TTP70260757, 288 m a.s.l., 16–IV–2011, M.C. Fdez.-Carvajal s.n. leg. (BC 917673).

Euphorbia angulata Jacq.

SPAIN. Girona: Ripollès, Sant Amanç, 1700 m a.s.l., 26–VI–1962, J. Vigo s.n. leg. (BC 149063); Ripollès, Sant Amanç, 1700 m a.s.l., 17–VIII–1962, J. Vigo s.n. leg. (BC 149079); Alt Empordà, Maçanet de Cabrenys, les Salines, DG79, 1000 m a.s.l., 13–VI–1983, J. Bou i Tomàs s.n. leg. (HGI 23850); Alt Empordà, Maçanet de Cabrenys, vers Tapis, DG79, 500 m a.s.l., 3–VII–1983, J. Bou i Tomàs s.n. leg. (HGI 23851).