Anales del Jardín Botánico de Madrid, Vol 60, No 1 (2002)

Detection and identification of phytoplasma DNA in symptomatic mushrooms of the genus Ramaria (O. Gomphales)

María P. Martín
Real Jardín Botanico, CSIC, Spain

Pablo P. Daniëls
Real Jardín Botanico, CSIC, Spain

Ester Torres
Laboratori de Sanitat Vegetal, DARP, Generalitat de Catalunya, Spain

M. Teresa Tellería
Real Jardín Botanico, CSIC, Spain


Using polymerase chain reaction assays with specific primers for amplifying phytoplasma 16S rDNA, the presence of phytoplasmas in Ramaria basidiomes with abnormal development has been detected for the first time in fungi. Phytoplasmas have not been detected in asymptomatic basidiomes. Sequence analyses based on parsimony and maximum likelihood place the phytoplasma in the Stolbur group.


16S rDNA; specific primers; Stolbur; fungi

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